Reverse genetics plasmid for cloning unstable Influenza A virus gene segments
نویسندگان
چکیده
منابع مشابه
A plasmid-based reverse genetics system for influenza A virus.
A reverse genetics system for negative-strand RNA viruses was first successfully developed for influenza viruses. This technology involved the transfection of in vitro-reconstituted ribonucleoprotein (RNP) complexes into influenza virus-infected cells. We have now developed a method that allows intracellular reconstitution of RNP complexes from plasmid-based expression vectors. Expression of a ...
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ژورنال
عنوان ژورنال: Journal of Virological Methods
سال: 2011
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2011.01.021